Practice experience of establishment of abdominal heart transplantation model combined with tail vein injection in mice (with video demonstration)
10.3969/j.issn.1674-7445.2019.02.009
- VernacularTitle:建立小鼠腹部心脏移植模型联合尾静脉注射的实践体会（附视频）
- Author:
Zhiye BAO
1
;
Jiayi ZHU
;
Qian JIAN
;
Qi PAN
;
Boqian LIU
;
Jingxu ZHANG
;
Keyi ZHAO
;
Caiyu YI
;
Hao LIU
Author Information
1. Department of Hepatobiliary Surgery, the First Hospital of China Medical University, Key Laboratory of Organ Transplantation of Liaoning Province, Shenyang 110001, China
- Publication Type:Research Article
- Keywords:
Abdominal heart transplantation;
Heart transplantation;
Tail vein injection;
Mouse;
Transplantation immunity;
Organ transplantation;
Rejection reaction;
Inflammatory cell
- From:
Organ Transplantation
2019;10(2):171-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To summarize the practice experience of establishing a stable abdominal heart transplantation model combined with tail vein injection in mice. Methods In the preliminary experiment, 50 pairs of donor and recipient Kunming mice received isotransplantation, 40 pairs of donor and recipient C57BL/6J mice underwent isotransplantation. In the formal experiment, 10 pairs of donor and recipient C57BL/6J mice received isotransplantation, 30 pairs of Balb/c mice as the donor and C57BL/6J mice as the recipient received allotransplantation. The time of each step of the heart transplantation (including harvesting and dressing of the donor heart, vascular anastomosis of the recipient, etc.) was recorded. The duration of transplanted heart beat and the survival time of the recipient was observed daily after operation. The time required for tail vein injection in the transplanted mice was recorded. Pathological examination of the transplanted heart was performed at 30 d after isotransplantation (n=5) and 7 d after allotransplantation (n=5). Results In the formal experiment, the success rate of heart transplantation was 90%. The harvesting and dressing time of donor heart was (13.9±0.6) min. The cold ischemia time of the recipient was (14.2±1.2) min. The vascular anastomosis time was (34.2±3.1) min. The total operation time was (86.6±5.4) min. Postoperatively, the transplanted heart of the mice undergoing isotransplantation survived longer than 100 d. Pathological examination at postoperative 30 d demonstrated only a slight amount of inflammatory cell infiltration. The survival time of the mice receiving allotransplantation was (7.2±0.5) d due to rejection reaction. At postoperative 7 d, pathological examination showed a large quantity of inflammatory cells infiltrating into the myocardium, manifested with acute cellular rejection. The success rate reached 90% after over 200 times of tail vein injection. Conclusions In this study, a stable mouse abdominal heart transplantation model is successfully established. The mouse models in the preliminary experiment can be utilized for tail vein injection.
