The importance of GDN motif in domain III and GXGXG motif in domain VI of L protein in replication of Nipah virus
- Author:
Siti Aishah Jalani
1
;
Nazlina Ibrahim
1
Author Information
1. Universiti Kebangsaan Malaysia
- Publication Type:Journal Article
- Keywords:
GDN motif of domain III;
GXGXG motif of domain VI;
conserved domain;
L gene Nipah virus minireplication system
- From:Malaysian Journal of Microbiology
2018;14(6):585-589
- CountryMalaysia
- Language:English
-
Abstract:
Abstracts:This study aims to determine the importance of conserved GDN motif in domain III and GXGXG motif in domain VI in Nipah virus (NiV) L protein. Four mutated L genes produced in an earlier study were inserted individually into plasmid pCITE. Optimised transfection protocol was successful in transfecting these plasmids, two helper plasmids (coding for N and P protein), NiV minigenome containing chloramphenicol acetyltransferase (CAT) reporter gene and T7 promoter. Successful in vitro transcription/translation in the NiV minireplicon system was monitored by CAT expression. In conclusion, GXGXG motif was important in the NiV minireplicon system but change of GDN motif does not affect L protein.
- Full text:20.2018my0492.pdf
