Purification and characterization of dehalogenase from Bacillus cereus SN1 isolated from cow dung
- Author:
Siti Nurul Fasehah Ismail
1
;
Mohamed Faraj Edbeib
2
;
Wan Mohd Khairul ikhsan Wan Seman
3
;
Mahzan Md. Tab
3
;
Farahayu Khairuddin
3
;
Amin Retnoningsih
4
;
Roswanira Abdul Wahab
1
;
FahrulHuyop
4
Author Information
1. Universiti Teknologi Malaysia
2. Bani Walid University
3. Malaysia Genome Institute
4. Universitas Negeri Semarang
- Publication Type:Journal Article
- Keywords:
Cow dung;
dehalogenase;
2,2-dichloropropionic acid;
Bacilluscereus SN1;
bioremediation
- From:Malaysian Journal of Microbiology
2018;14(3):244-253
- CountryMalaysia
- Language:English
-
Abstract:
Aims:This study was aimed to characterize a dehalogenase derived from Bacillus cereus SN1 isolated from cow dung.
Methodology and results:Cell-free extract of Bacillus cereus SN1 was purified using ion exchange and gel filtration chromatography. Fraction B2 of gel filtration gave the highest enzyme specific activity (0.155 μmol CI¯/min/mg). The results of SDS-PAGE showed the enzyme was 25 kDa in size. The enzyme reached its optimum activity at 30 °C at pH 6, and was inhibited by Mercury(II) sulfate (HgSO4). The Km and kcat values were 0.2 mM and 1.22/sec, respectively. The partial dehalogenase gene sequence was amplified using Group I dehalogenase primers. The amplified gene sequence was designated as DehSN1.
Conclusion, significance and impact of study:Dehalogenase from Bacillus cereus strain SN1 revealed new characteristics of dehalogenase protein. The findings indicated that the DehSN1 dehalogenase is a promising candidate for further studies as a bioremediation agent for agricultural applications.
- Full text:20.2018my0449.pdf
