- VernacularTitle:一种检测安丝菌素效价的生物检定方法——木霉平板法
- Author:
Shu-fen LI
1
;
Jing WANG
1
;
Gui-zhi SUN
1
;
Tao ZHANG
1
;
Li-yan YU
1
;
Bing-ya JIANG
1
;
Lin-zhuan WU
1
Author Information
- Publication Type:Research Article
- Keywords:
ansamitocin;
bioassay;
italic>Trichoderma CPCC
400749 - From: Acta Pharmaceutica Sinica 2019;54(12):2340-2344
- CountryChina
- Language:Chinese
-
Abstract:
This paper describes a bioassay method for the determination of ansamitocin titers. A fungal strain sensitive to ansamitocin was classified to the genus
Trichoderma based on phylogenetic analysis of its ITS sequence, and designated asTrichoderma CPCC 400749. PDA plates ofTrichoderma CPCC 400749 were prepared to assay ansamitocin titers ofActinosynnema pretiosum ATCC 31565. The titers were consistent with those determined by HPLC. The bioassay method may have the potential use in high-throughput screening forActinosynnema pretiosum mutants with improved ansamitocin titers.