Bioassay of ansamitocin by <italic>Trichoderma</italic> plate

VernacularTitle:一种检测安丝菌素效价的生物检定方法——木霉平板法
Author: Shu-fen LI ¹ ; Jing WANG ¹ ; Gui-zhi SUN ¹ ; Tao ZHANG ¹ ; Li-yan YU ¹ ; Bing-ya JIANG ¹ ; Lin-zhuan WU ¹
Author Information

1. NHC Key Laboratory of Biotechnology of Antibiotics, CAMS Key Laboratory of Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Publication Type:Research Article
Keywords: ansamitocin; bioassay; italic>Trichoderma CPCC400749
From: Acta Pharmaceutica Sinica 2019;54(12):2340-2344
CountryChina
Language:Chinese
Abstract: This paper describes a bioassay method for the determination of ansamitocin titers. A fungal strain sensitive to ansamitocin was classified to the genus Trichoderma based on phylogenetic analysis of its ITS sequence, and designated as Trichoderma CPCC 400749. PDA plates of Trichoderma CPCC 400749 were prepared to assay ansamitocin titers of Actinosynnema pretiosum ATCC 31565. The titers were consistent with those determined by HPLC. The bioassay method may have the potential use in high-throughput screening for Actinosynnema pretiosum mutants with improved ansamitocin titers.