Bioassay method for Compound Danshen Tablets based on a platelet aggregation inhibition test
10.16438/j.0513-4870.2019-0526
- VernacularTitle:复方丹参片体外抑制血小板聚集的生物活性测定法
- Author:
Yu-dong GUO
1
;
Yu-chi HU
1
;
Cun-ran CAO
1
;
Zhi-bin WANG
2
;
Ze-ping ZUO
1
;
Yang GAO
1
;
Tan-yu LOU
2
;
Bei-bei MA
2
Author Information
1. Beijing Key Laboratory of Analysis and Evaluation on Chinese Medicine, Beijing Institute for Drug Control, Beijing 102206, China
2. Beijing University of Chinese Medicine, Beijing 100102, China
- Publication Type:Research Article
- Keywords:
Compound Danshen Tablet;
platelet aggregation;
bioassay method;
quality control
- From:
Acta Pharmaceutica Sinica
2019;54(12):2184-2188
- CountryChina
- Language:Chinese
-
Abstract:
A bioassay method for inhibiting platelet aggregation in vitro was established to quantify the pharmacological effects of Compound Danshen Tablets and support its quality control. The inhibition of platelet aggregation in rabbit plasma in vitro by Compound Danshen Tablets was used as the experimental system. The titer was calculated by using the method of dose-response parallel lines. As a result, a bioassay for the inhibition of platelet aggregation in vitro by Compound Danshen Tablets was established. Linearity was good in the concentration range of 0.128 g·mL-1 to 0.205 g·mL-1, and the titer of standard Compound Danshen tablets was 7 659 U·g-1 according to the titer definition. This in vitro assay was simple, reliable, reproducible and convenient. The activity of Compound Danshen Tablets in inhibiting platelet aggregation was quantified by potency assay and the quality of different batches was evaluated. The method can be applied for the quality control of Compound Danshen Tablets.
