Measurement of antiplatelet aggregation and potency of hirudo
10.16438/j.0513-4870.2019-0147
- VernacularTitle:水蛭抗血小板聚集生物效价检测方法研究
- Author:
Xuan WANG
1
,
2
;
Qi-chao GAN
3
;
Jing-yan SHI
3
;
Huang-wan-yin HU
4
;
Jun-jie HAO
4
;
Guo-song WU
4
;
Qian GAO
1
;
Xiang LI
1
;
Ming NIU
4
;
Jia-bo WANG
4
;
Li MA
1
;
Xiao-he XIAO
4
Author Information
1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
2. China Military Institute of Chinese Medicine, Integrative Medical Center, Fifth Medical Center, General Hospital of Chinese PLA, Beijing 100039, China
3. Chongqing Duoputai Pharmaceutical Co., Ltd., Chongqing 401420, China
4. China Military Institute of Chinese Medicine, Integrative Medical Center, Fifth Medical Center, General Hospital of Chinese PLA, Beijing 100039, China
- Publication Type:Research Article
- Keywords:
hirudo;
promoting blood circulation;
anti-platelet aggregation;
potency;
quality evaluation
- From:
Acta Pharmaceutica Sinica
2019;54(12):2178-2183
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this work was to develop a bioassay to quantify the antiplatelet aggregation activity of hirudo for quality evaluation and control. Antithrombin activity of hirudo extracted by high temperature decoction was determined by thrombin titration. Antiplatelet aggregation activity of hirudo was determined through pharmacodynamic experiments in vitro and in vivo using a bioassay we developed for quantifying inhibition of platelet aggregation. Methodological investigation was carried out and the titers of 12 batches of hirudo samples were determined. During the experiment, the disposal of animals is in accordance with the ethical standards of animal experiments. The results showed that the antithrombin activity of hirudo decocted at high temperature decreased significantly and almost lost its activity. Hirudo inhibited platelet aggregation and results in vivo and in vitro were consistent. These assays were employed to test 12 batches of hirudo. The results demonstrated that the biopotency of 12 batches was 113.49, 96.13, 121.22, 127.33, 83.48, 108.72, 131.41, 127.95, 76.90, 126.27, 132.89 and 573.53 U·mg-1. The method was reliable and reproducible and can be used to assess the quality of hirudo.
