Study on the surface fungus diversity of commercially Platycladi semen based on high-throughput sequencing technology
10.16438/j.0513-4870.2019-0512
- VernacularTitle:基于高通量测序技术对市售柏子仁表面真菌多样性的研究
- Author:
Ting LI
1
;
Dan JIANG
1
;
Xiao-song HU
1
;
Xiao-xi CHANG
1
;
Zhen XU
1
;
Ying GONG
2
;
Guo-dong HUA
2
;
Chun-sheng LIU
1
Author Information
1. School of Chinese Medica, Beijing University of Chinese Medicine, Beijing 102488, China
2. Department of Pharmacy, Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China
- Publication Type:Research Article
- Keywords:
high throughput sequencing;
Platycladi semen;
italic>Aspergillus flavus;
fungi;
iversity
- From:
Acta Pharmaceutica Sinica
2019;54(11):2100-2105
- CountryChina
- Language:Chinese
-
Abstract:
The structural composition of the surface fungal community of commercially Platycladi semen was analyzed to reveal the surface fungal biodiversity and structural differences. Platycladi semen was collected from Henan, Shandong and Hong Kong, their DNA was extracted, ITS fragments in DNA were amplified by PCR. Miseq was sequenced on Illumina Hiseq 2500 platform after the PCR products were qualified for quality inspection. The sequence OTU cluster was obtained and bioinformatics analysis was carried out. Microbial communities were not observed in the eyes of the Platycladi semen in the three regions. Sequencing results showed that the surface microbial community had high biodiversity, but there were significant differences in species composition. Seven samples o Platycladi semen obtained 345 947 valid sequences, which were divided into 267 OTUs, 3 phylums. 18 classes, 40 orders, 82 families, 120 genus, 191 species fungi. At the genus level, Aspergillus is the dominant species, accounting for the highest proportion, reaching (93.36 ± 6.01)%. Seven samples were contaminated by Aspergillus flavus, and the pollution levels were 14.58%, 15.98%, 17.64%, 16.44%, 0.97%, 23.39% and 18.86%. Except sample No. 5, Aspergillus cibarius was the most abundant, the other six samples were Aspergillus niger, Aspergillus fumigatus and Aspergillus flavus as the core microflora. By analyzing the diversity of fungi distribution in different habitats, we can fully understand the fungi on the surface of Platycladi semen, lay a foundation for early risk warning of Aspergillus flavus contamination and its aflatoxin contamination, and provide a theoretical basis for the quality and safety of Platycladi semen.
