Fingerprint analysis and multi-component determination of Leonurus japonicus by high performance liquid chromatography
10.16438/j.0513-4870.2019-0407
- VernacularTitle:益母草HPLC指纹图谱及多成分含量测定方法研究
- Author:
Chen SUN
1
,
2
;
Zhe-yu XIE
3
;
Yong-jiang WU
1
Author Information
1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China
3. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China
- Publication Type:Research Article
- Keywords:
italic>Leonurus japonicus;
fingerprint;
multi-component determination
- From:
Acta Pharmaceutica Sinica
2019;54(11):2059-2063
- CountryChina
- Language:Chinese
-
Abstract:
An HPLC fingerprint and multi-component determination method of Leonurus japonicus was established for comprehensive evaluation and quality control of Leonurus japonicus. The sample was incubated in 70% ethanol in a water bath for 2 h, and the extract was analyzed by HPLC using Kromasil C18 column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution. The flow rate was 1.0 mL·min-1. The temperature of column was 30 ℃. The detection wavelength was 280 nm. HPLC fingerprint of characteristic components of Leonurus japonicus was established. There were 12 common peaks among 25 batches of samples, and 5 of them were identified and determined. Syringic acid, leonurine hydrochloride, rutin, hyperoside or isoquercitrin showed a good linearity in the ranges of 0.426 1-85.22 ng (r = 0.999 9), 7.948-1 590 ng (r = 0.999 3), 10.20-2 040 ng (r = 1.000 0), 2.018-403.6 ng (r = 0.999 9), or 8.704-1 741 ng (r = 0.999 9), respectively. The average recoveries were 99.0%, 97.6%, 97.4%, 96.9% and 98.5% with RSD of 1.1%, 1.8%, 1.4%, 1.5% and 1.3%, respectively. The HPLC characteristic fingerprint of Leonurus japonicus was specific, and this method can simultaneously determine the content of 5 components.
