Comparative study on the effect of Danggui-Chuanxiong herb pair on vasoactive substances and adhesion molecules in the serum of acute blood stasis in rats using PLS-DA and multi-attribute comprehensive index methods
10.16438/j.0513-4870.2019-0227
- VernacularTitle:基于PLS-DA和多指标综合指数法研究当归-川芎药对对急性血瘀大鼠血清中血管活性物质和黏附分子的影响
- Author:
Wei-xia LI
1
;
Xiao-yan WANG
1
;
Jin-fa TANG
1
,
2
;
Shu-qi ZHANG
3
;
Yan WANG
3
;
Hui ZHANG
1
;
Xian-lei LIU
1
;
Xiang-le MENG
1
;
Xue-lin LI
1
,
2
;
Yu-ping TANG
4
Author Information
1. Henan Province Engineering Laboratory for Clinical Evaluation Technology of Chinese Medicine, the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, China
2. Henan University of Chinese Medicine, Zhengzhou 450046, China
3. Henan University of Chinese Medicine, Zhengzhou 450046, China
4. Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, Shanxi University of Chinese Medicine, Xi'an 712046, China
- Publication Type:Research Article
- Keywords:
Danggui;
Chuanxiong;
herb pair;
partial least squares discrimination analysis;
multi-attribute comprehensive index method;
activating blood circulation to dissipate blood stasis
- From:
Acta Pharmaceutica Sinica
2019;54(11):1909-1917
- CountryChina
- Language:Chinese
-
Abstract:
We evaluated the effects of Danggui-Chuanxiong (GX) herb pair with different proportions (1∶0, 3∶2, 1∶1, 2∶3, 0∶1) and preparation methods (water extract W, alcohol extract A, and water-alcohol extracts WA) on vasoactive substances and endothelial cell adhesion molecules in the serum of acute blood stasis in rats. An acute blood stasis model was co-replicated by ice water bath and subcutaneous injection of epinephrine hydrochloride in rats. The expressions of vasoactive substances (arachidonic acid metabolites, coagulation-fibrin system index) and adhesion molecules in the serum were detected by enzyme linked immunosorbent assay method; the Spearman method was used to analyze the correlation of those detection indicators; the partial least squares-discriminant analysis and multi-attribute comprehensive index method were used to comprehensively evaluate the total effect of GX herb pair samples with different proportions and preparation methods on vasoactive substances and adhesion molecules. The experimental scheme was approved by the Animal Experimental Ethics Committee of the First Affiliated Hospital of Henan University of Chinese Medicine. The results showed that GX 1∶1_WA had the strongest effect on the improvement of vasoactive substances and adhesion molecules in the serum of acute blood stasis in rats (the total effect value was 6.96). When extraction method was same, the overall effect of GX 1∶1 had better effect than that of other proportions; when the proportion of GX was same, the total effects of GX_WA and GX_A were better than GX_W. The combination of Danggui and Chuanxiong can significantly improve the expressions of vasoactive substances and adhesion molecules in the serum of blood stasis in rats. But the action strength of GX herb pairs was different when the proportions and preparations of GX herb pair were different. These findings provide a basis for clinical rational application of GX herb pair, and lay the foundation for in-depth research on GX herb pair for treatment of blood stasis related diseases.
