Ginsenoside CK induces apoptosis of human liver cancer SMMC-7721 cells through inhibition of TGF-β1/Smads signaling pathway
10.16438/j.0513-4870.2019-0088
- VernacularTitle:人参皂苷CK通过抑制TGF-β1/Smads通路诱导人肝癌SMMC-7721细胞凋亡的作用
- Author:
Yan YAN
1
;
Si-lin ZHANG
1
;
Jia-xin CHEN
1
;
Wen-jun JIAO
1
;
Xue-wu ZHANG
1
Author Information
1. Yanbian University Medical College, Yanji 133002, China
- Publication Type:Research Article
- Keywords:
hepatocellular carcinoma;
ginsenoside CK;
TGF-β1/Smads signaling;
apoptosis
- From:
Acta Pharmaceutica Sinica
2019;54(9):1606-1611
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to investigate apoptosis induction of ginsenoside compound K (ginsenoside CK) in human liver cancer SMMC-7721 cells and the involvement of TGF-β1/Smads signaling pathway. MTT assay was used to detect cell viability following ginsenoside CK treatment in SMMC-7721 cells. Annexin V-FITC/PI assay was used to detect apoptosis. After ginsenoside CK, or TGF-β1/Smads pathway activator TGFβ1 and inhibitor LY2109761 treatment, the TGF-β1/Smads pathway proteins and apoptosis proteins were detected by Western blot. The results showed that ginsenoside CK inhibited the proliferation of SMMC-7721 cells in a dose- and time-dependent manner. Annexin V-FITC/PI showed that ginsenoside CK induced apoptosis in SMMC-7721 cells. Meanwhile, ginsenoside CK inhibited the expression of Smad2/3, p-Smad2/3, Smad4, but promoted Smad7 expression, cleavage of caspase-3 and down-regulated Bcl-2/Bax. Compared with TGFβ1 treatment alone, levels of Smad2/3, p-Smad2/3, Smad4 and the ratio of Bcl-2/Bax were down-regulated, whereas Smad7 or cleaved caspase-3 was up-regulated in the ginsenoside CK+TGF-β1 group. In addition, Smad2/3, p-Smad2/3 and Smad4 expression were decreased in LY2109761 group. Compared with LY2109761 group, cleaved caspase-3 expression and Bcl-2/Bax have no significant change in ginsenoside CK+LY2109761 group. Taken together, our results showed that ginsenoside CK induced apoptosis in SMMC-7721 cells, and such induction is related to inhibiting TGF-β1/Smads signaling pathway.
