Construction and preliminary study of multifunctional drug delivery system based on hollow mesoporous copper sulfide nanoparticles
10.16438/j.0513-4870.2019-0253
- VernacularTitle:基于中空介孔硫化铜的多功能纳米递药系统的构建及初步研究
- Author:
Ya-ting WANG
1
;
Zhen-hua ZHANG
1
;
Qian-hua FENG
2
;
Meng LIN
1
Author Information
1. School of Basic Medical Sciences
2. School of Pharmaceutical Sciences, Zhengzhou University, Zhenzhou 450001, China
- Publication Type:Research Article
- Keywords:
nanoparticle;
copper sulfide;
targeting;
photodynamic therapy;
photothermal therapy
- From:
Acta Pharmaceutica Sinica
2019;54(8):1502-1508
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to develop multifunctional drug delivery system based on hollow mesoporous copper sulfide (HMCuS) nanoparticles. This type of nanoparticles is expected to achieve the synergistic treatment of tumor by targeted phototherapy and chemotherapy. The carrier was synthesized by a substitution method, and the anti-cancer drug doxorubicin (DOX) was loaded and then modified by hyaluronic acid (HA) to prepare the anti-cancer drug system DOX/HMCuS-HA. The results suggested that DOX/HMCuS-HA presented uniform spherical structure, with the drug loading efficiency of 33.6%, the particle size and zeta potential being 113.8 ± 6.9 nm and 18.4 ± 2.8 mV, respectively. When 100 μg·mL-1 HMCuS was irradiated under 808 nm laser (2 W·cm-2) for 8 min, the temperature can heat up 51 ℃, demonstrating high photothermal conversion efficacy. Electron spin resonance (ESR) tests and methylene blue degradation experiments showed that HMCuS nanoparticles could simultaneously produce hydroxyl radical (•OH) mediated photodynamic therapy. In addition, HA was responsible for minimizing premature drug release and increasing tumor targeting efficiency by acting as a smart gatekeeper with tumor specific targeting moiety. In vitro drug release experiments showed that the coated HA could be degraded by intracellular lysosomal enzyme hyaluronidase, which facilitated DOX release. The acidic micro-environment of tumor cell and external near infrared (NIR) stimulus could trigger further release of DOX from the nanoparticles. These results point to a new strategy for timely and effective anti-tumor treatment.
