The black phosphorus quantum dots-based photothermal effect on dendritic cells activation
10.16438/j.0513-4870.2019-0317
- VernacularTitle:基于黑磷量子点的光热效应在树突状细胞激活中的作用
- Author:
Xin-yu YE
1
,
2
;
Lin MEI
3
,
4
Author Information
1. School of Life Sciences, Tsinghua University, Beijing 100084, China
2. Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
3. Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
4. School of Pharmaceutical Sciences (Shenzhen), Sun Yat-Sen University, Shenzhen 510275, China
- Publication Type:Research Article
- Keywords:
immunotherapy;
tumor vaccine;
black phosphorus quantum dot;
cell membrane;
endritic cell
- From:
Acta Pharmaceutica Sinica
2019;54(7):1297-1302
- CountryChina
- Language:Chinese
-
Abstract:
Immunotherapy is the most active research area for cancer treatment. Tumor vaccine is one of the most developed aspects of cancer immunotherapy. Though tumor vaccine has made many breakthrough, it still faces many challenges. In this study, we coated the black phosphorus quantum dots (BPQDs) with cancer cell membrane to create a nanoparticle named BPQD-CCNVs. The BPQD-CCNVs were incubated with bone marrow-derived dendritic cells and irradiated with 808 nm infrared light. We tested the expression level of CD80, CD86 and MHC II of dendritic cells by flow cytometry after irradiation. All animal experiments approved by the Animal Experiments Ethical Committee of Tsinghua University. The results showed that the rise of medium's temperature caused by the photothermal effect of BPQDs could upregulate the expression of CD80, CD86 and MHC-II on dendritic cell surface. Based on these, we conclude that near infrared irradiation can stimulate the activation of dendritic cells. Our study may have provided a new strategy for tumor vaccine development.
