Analysis of transcriptomes to explore genes contributing to iridoid biosynthesis in Gentiana waltonii and Gentiana robusta (Gentianaceae)
10.16438/j.0513-4870.2019-0082
- VernacularTitle:藏药解吉基原植物转录组及其环烯醚萜苷类生物合成相关基因的探究
- Author:
Liang-hong NI
1
;
Zhi-li ZHAO
1
;
Jin-rong WU
1
;
Dorje GAAWE
2
;
Ma MI
2
Author Information
1. School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Tibetan Traditional Medical College, Lhasa 850000, China
- Publication Type:Research Article
- Keywords:
italic>Gentiana waltonii;
italic>Gentiana robusta;
transcriptome;
iridoids;
biosynthesis;
metabolism pathway
- From:
Acta Pharmaceutica Sinica
2019;54(5):944-953
- CountryChina
- Language:Chinese
-
Abstract:
The roots and flowers of Gentiana waltonii and Gentiana robusta are used as Tibetan herb Jie-Ji in traditional Tibetan medicine, with iridoids as the main active ingredient and index components. To study the pathway of iridoid biosynthesis, roots, stems, leaves and flowers of G. waltonii and G. robusta were subjected to a high-throughput transcriptomic sequencing analysis by Illumina HiseqXTen. After removing insignificant reads and de novo splicing, 79 455 and 78 466 unigenes were obtained from G. waltonii and G. robusta respectively, with average length as 834 bp and 862 bp. The unigene GO functions could be divided into three categories of 65 branches. The unigenes were aligned in KOG database and were classified into 25 classes according to function. In KEGG database, 315 and 340 unigenes of G. waltonii and G. robusta were implicated in 20 standard secondary metabolic pathways, respectively. Furthermore, 80 and 57 unigenes of the two species were analyzed to encode 24 key enzymes in the pathway related to iridoid biosynthesis. There were differences in gene expression among different organs. Based on sequence data, significant amounts of SSRs, SNPs and InDels were detected in each dataset. This study provides a platform for further development of molecular markers, excavation of functional genes, and research into metabolic pathways and their regulatory mechanism within G. waltonii and G. robusta.
