Synthesis, in vitro evaluation and in vivo imaging of a highly selective hydrazine bioluminescent probe based on luciferin-luciferase system
- VernacularTitle:基于luciferin-luciferase体系的肼生物发光探针的合成、体外评价及活体成像
- Author:
Feng XU
1
;
Ao HAI
2
;
Ya-ru MA
2
;
Li-ying QIU
2
;
Min-yong LI
1
;
Lü-pei DU
1
Author Information
- Publication Type:Research Article
- Keywords:
hydrazine;
bioluminescent probe for hydrazine;
italic>D-luciferin;
small molecule probe;
italic>in vivo
imaging - From: Acta Pharmaceutica Sinica 2019;54(5):886-891
- CountryChina
- Language:Chinese
-
Abstract:
A highly sensitive and selective bioluminescent probe for hydrazine (BPH) was designed, synthesized and evaluated for detection of hydrazine
in vitro andin vivo . BPH was designed to include a specific recognition group (acetyl) of hydrazine at an appropriate modification site of the optical reporter hydroxyluciferin (D -luciferin), which showed excellent performance both in selectivity and sensitivity to hydrazine. The results showed that the bioluminescent probe BPH developed in this study is an innovative and widely applicable tool for detecting hydrazine in complex natural environment or in animals.
