Recent advances in enzyme inhibitor screening based on mass spectrometry
10.16438/j.0513-4870.2018-1132
- VernacularTitle:质谱技术在酶抑制剂筛选中的研究进展
- Author:
Li ZHU
1
,
2
;
Xuan ZOU
1
,
2
;
Tang-rong LIU
1
,
2
;
Ruo-nan LIU
1
,
2
;
Zhe XU
1
,
2
,
3
Author Information
1. Key Laboratory of Marine Drugs, Chinese Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
2. Laboratory for Marine Drugs and Bioproducts, Innovation Center for Marine Drugs Screening and Evaluation, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
3. Marine Biomedical Research Institute of Qingdao, Qingdao 266071, China
- Publication Type:Research Article
- Keywords:
mass spectrometry;
rug discovery;
inhibitor screening;
enzyme assay
- From:
Acta Pharmaceutica Sinica
2019;54(5):818-827
- CountryChina
- Language:Chinese
-
Abstract:
Enzymes play crucial functional roles in all biological processes. Enzymatic inhibitors can regulate enzyme activity and may become the starting point for drug discovery. Mass spectrometry (MS) has the advantage for rapid qualitative and quantitative analyses of compounds and enzyme reactions, emerging as an important analytical tool in enzyme inhibitor screening assay for drug discovery. This review will highlight recent advances in the inhibitor screening assay using MS and related techniques, including frontier affinity chromatography, immobilized enzyme beads, ultrafiltration, surface plasmon resonance, capillary electrophoresis and microfluidic chips. The existing MS methods for screening enzyme inhibitor were divided into two types: affinity screening and activity screening.
