Experimental study on the effect of Sishen Wan on intestinal flora in rats with diarrhea-type irritable bowel syndrome
10.16438/j.0513-4870.2018-0920
- VernacularTitle:四神丸对腹泻型肠易激综合征大鼠肠道菌群影响的实验研究
- Author:
Jia-xing LIU
1
;
Yan-li WANG
2
;
Yu LI
3
;
Di-xin ZOU
2
;
Dun-fang WANG
2
;
Xu-ran MA
2
;
Hong-xin SONG
2
;
Wei-peng YANG
2
;
Hai-nan WANG
4
Author Information
1. School of Pharmacy, Guizhou University, Guiyang 550025, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
3. Beijing University of Chinese Medicine, Beijing 100029, China
4. State Drug Administration, Beijing 100037, China
- Publication Type:Research Article
- Keywords:
Sishen Wan;
Er Shen Wan;
Wuweizisan;
iarrhea-predominant irritable bowel syndrome;
intestinal flora;
high-throughput sequencing
- From:
Acta Pharmaceutica Sinica
2019;54(4):670-677
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of Sishen Wan (SSW) on intestinal flora in diarrhea-predominant irritable bowel syndrome (IBS-D) rats and explore the efficacy of this regiment for improving IBS-D, we divided 45 SPF male SD rats randomly into control, disease, SSW, Ershen Wan (ESW) and Wuweizasan (WWZS) groups. The spleen-kidney-yang deficiency type IBS-D rat model was prepared by a composite factor and administered for 14 days. After collecting the feces of the rats, total DNA was extracted from the stool samples. Primers were designed based on the 16S r RNA V3 to V4 regions of the bacteria, and used for high-throughput sequencing with the Illumina Miseq platform. We found that SSW can effectively reduce the diarrhea index (P<0.05) and reduce the high sensitivity of intestinal tract (P<0.05) of IBS-D rats. The principal component analysis (PCA), principal co-ordinates analysis (PCoA) and non-metric multidimensional scale analysis (NMDS) based on the Beta diversity distance showed that there were significant differences in the composition of the gut microbiota among the five groups (P<0.05). The disease group has the lowest in abundance, uniformity and diversity of gut microbiota. Compared with the control group, the disease group showed a significant increase in Proteobacteria, Actinobacteria, Veillonococcus and Mycoplasma (P<0.05), but a significant reduction in Pleaverella (P<0.05). Compared with the disease group, SSW administration caused significant reduction in the Proteobacteria and Mycoplasma (P<0.05), but significant increases of Clostridium, Turicibacter and Romboutsia (P<0.05). Our study shows that SSW has the potential as a therapeutic regiment for treatment of IBS-D due to partial regulation of the intestinal flora. In addition, there is a synergy between ESW and WWZS.
