Quantification of norfloxacin in human plasma by LC-MS/MS and validation of the analytical method
10.16438/j.0513-4870.2018-0913
- VernacularTitle:LC-MS/MS法定量测定人血浆中诺氟沙星浓度的分析方法研究及其应用
- Author:
Shan-shan LIU
1
,
2
;
Ya-jie GENG
3
;
Yuan-yuan XIA
3
;
Jing-yuan LIU
3
;
Guan-li WEI
3
;
Duan-yun SI
3
Author Information
1. Tianjin Medical University Graduate School, Tianjin 300070, China
2. State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China
3. State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China
- Publication Type:Research Article
- Keywords:
norfloxacin;
LC-MS/MS;
pharmacokinetics
- From:
Acta Pharmaceutica Sinica
2019;54(4):695-700
- CountryChina
- Language:Chinese
-
Abstract:
A LC-MS/MS method for quantification of norfloxacin in human plasma had been developed. This method was applied to the pharmacokinetics study of norfloxacin in the human. The plasma sample was precipitated by methanol and ciprofloxacin was used as the internal standard (IS). Chromatographic separation was performed on a Symmetry® C18 column(100 mm×4.6 mm, 5 μm). Mobile phase contains 0.3% formic acid and 5% methanol in deionized water at a flow rate of 0.45 mL·min-1. Norfloxacin and ciprofloxacin (IS) were ionized with an ESI source and operated in positive ion mode. The detected ions were m/z 320.3→302.1 (norfloxacin), m/z 332.3→314.1 (ciprofloxacin). This LC-MS/MS method yielded a linearity over the range of 10-1 000 ng·mL-1 with the lower limit of quantitation (LLOQ) of 10 ng·mL-1. The intra and inter-assay precisions (RSD%) were within the range of 2.64%-7.23% and the accuracy (RE%) was less than ±5.00%. The pharmacokinetic parameters tmax, Cmax, AUC0-t, and t1/2 were 1.28±0.364 h, 627±171 ng·mL-1, 2 938±850 h·ng·mL-1, and 6.01±1.36 h, respectively. This LC-MS /MS method was proven simple, sensitive, rapid and suitable for pharmacokinetics study of norfloxacin in the human and Approved by the Medical Ethics Committee of Liuzhou Workers' Hospital.
