Comparative study on the effects of different proportions of ginkgo ketoester and donepezil on anti-dementia based on principal component analysis and multi-attribute comprehensive index methods
10.16438/j.0513-4870.2018-0885
- VernacularTitle:基于主成分分析和多指标综合指数法对银杏酮酯-多奈哌齐不同量比抗痴呆作用比较研究
- Author:
Jing ZHANG
1
;
Xu-qin SHI
1
;
Gui-sheng ZHOU
1
;
Ya-jie TAN
1
;
Jia-qian CHEN
1
;
Hui-juan TAO
1
;
Zong-jin PU
1
;
Pei LIU
1
;
Yue ZHU
1
;
Da-wei QIAN
1
;
Yu-ping TANG
1
,
2
;
Jin-ao DUAN
1
Author Information
1. Jiangsu Provincial Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
2. Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, Shaanxi University of Chinese Medicine, Xi'an 712046, China
- Publication Type:Research Article
- Keywords:
flavonoid;
ginkgolide;
onepezil;
compatibility;
ementia;
principal component analysis;
multi-attribute comprehensive index method
- From:
Acta Pharmaceutica Sinica
2019;54(4):660-669
- CountryChina
- Language:Chinese
-
Abstract:
The combination of ginkgo ketoester tablet - donepezil (GD) is a popular combination commonly used in clinic for the treatment of Alzheimer's disease. To evaluate the learning and memory improving ability of different proportions of the two drugs. We optimized the ratio of GD for treatment of dementia using a mouse model. Dementia was induced by multiple neuronal damages in mice. The experimental protocols were approved by the Animal Experimental Ethical Committee of Nanjing University of Chinese Medicine and all the procedures were strictly conducted in accordance with ethical principle of animal use and care. Morris water maze, brain hematosylin-eosin staining and the changes of the neurotransmitters and related enzymes in the plasma or brain tissues were tested to determine the effect of GD on dementia mice. The results showed that the dementia mice were significantly different from the normal group in terms of behavior, pathological sections and related indicators. Compared to the dementia mice, partial administration groups could improve learning and memory ability as well as indexes in the blood and brain tissues. Both the principal component analysis and multi-attribute comprehensive index methods were used to comprehensively evaluate the total effect of GD on anti-dementia. The results showed that the combination of two drugs at the dose of 0.5 to 1 times was in a dose-effect relationship, and the dose of 1 (the clinical equivalent) had the best treatment effect. Then based on the optimal dose, GD 1∶1 had best effect, which was consistent with the clinical use of two drugs. This provides scientific basis for more effective application of the compatibility between ketoester tablet and donepezil for modern clinic medicine.
