Rg1 alleviates the cholestatic liver fibrosis throughregulation of NF-κB/VCAM-1
10.16438/j.0513-4870.2018-0699
- VernacularTitle:人参皂苷Rg1调节NF-κB/VCAM-1减轻胆汁淤积性肝纤维化
- Author:
Piao LUO
1
,
2
;
Shi-feng CHU
3
;
Ye PENG
1
,
2
;
Zhao ZHANG
3
;
Nai-hong CHEN
1
,
2
Author Information
1. College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China
2. State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Neuroscience Center, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
3. State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Neuroscience Center, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
- Publication Type:Research Article
- Keywords:
ginsenoside Rg1;
cholestasis;
liver fibrosis;
vascular cell adhesion molecule 1;
hepatitis
- From:
Acta Pharmaceutica Sinica
2019;54(2):321-328
- CountryChina
- Language:Chinese
-
Abstract:
A mouse model of cholestatic liver fibrosis was established by bile duct ligation (BDL) method. The effect of ginsenoside Rg1 in the disease progress and the mechanism of cholestatic liver fibrosis are investigated in this mouse model. All animal experiments in this paper have been approved by the Unit Ethics Committee. Analysis of serum biochemical indicators and pathological sections assessed liver function, liver damage and fibrosis in mice. Immunohistochemistry and Western blot assays were used to detect vascular cell adhesion molecule-1 (VCAM-1) in BDL-induced mice. Nuclear factor-κB (NF-κB) and inflammatory factors were detected to investigate related mechanism of Rg1. The results showed that expression of VCAM-1 was up-regulated and peaked at 7 days, followed by decreased expression, but still efficiently expressed compared to the sham-operated group. Compared with the model group, 40 mg·kg-1·d-1 Rg1 treatment reduced serum aspartate transaminase (AST), alanine transaminase (ALT) and total bilirubin (T.Bili) levels (P<0.05 or P<0.01) and liver function damage,alleviated BDL-induced liver fibrosis, significantly down-regulated the expression of VCAM-1 (P<0.05), and inhibited the inflammatory response. In addition, Rg1 significantly reduced NF-κB p65 level in the cellular nucleus (P<0.05). This study demonstrates that VCAM-1 is dynamically altered during BDL-induced liver fibrosis. Rg1 could dampen inflammation and alleviate cholestatic liver fibrosis via regulation of the NF-κB/VCAM-1 pathway. The results provide an experimental basis for Rg1 application for treating liver fibrosis.
