Determination of six saikosaponins in Bupleurum chinense DC. samples collected from different producing areas at different harvest time with different processing methods
10.16438/j.0513-4870.2018-0544
- VernacularTitle:北柴胡不同产地、不同采收期和不同炮制品中6种柴胡皂苷的含量测定
- Author:
Hui-ping HOU
1
;
Shi-bo ZHAO
2
;
Kang-ping YU
1
;
Qi WANG
1
;
Hua-rong XU
1
;
Kai-shun BI
1
;
Qing LI
1
Author Information
1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
2. Shenyang No.2 High School, Shenyang 110016, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Bupleurum chinense DC.;
saikosaponin;
harvest time;
processing;
HPLC;
assay
- From:
Acta Pharmaceutica Sinica
2018;53(11):1887-1893
- CountryChina
- Language:Chinese
-
Abstract:
An HPLC method was established for the simultaneous determination of saikosaponin a, b2, c, d, e, f of Bupleurum chinense DC. in order to study the content difference of saikosaponins in different producing areas, different harvest time and different processed products of Bupleurum chinense DC. The Agela Venusil MP C18 (4.6 mm×250 mm, 5 μm) column was used with a gradient elution of acetonitrile-water at the wavelength of 210 and 254 nm with the flow rate of 1.0 mL·min-1 and the column temperature at 30℃. Based on the content of six kinds of saikosaponins, the differences of saikosaponins in four producing areas, eight harvest periods and 11 processing methods of Bupleurum chinense DC. were systematically studied. The results showed that the content of saikosaponins in Bupleurum chinense DC. was higher in May and August of Liaoning, Shaanxi and Gansu, but only in August from Shanxi in the four producing areas. The content of saikosaponins in 11 processed products was as follows:raw product > bran-stir-fried product > stir-fried product > wine-moistened product > turtle blood-stir-fried product > bran-wine-stir-fried product > wine-stir-fried product > vinegar-moistened product > turtle blood-wine-stir-fried product > vinegar-stir-fried product > honey-stir-fried product > honeymoistened product.
