Study on Radix Scrophulariae for the treatment of hyperthyroidism rat model of yin deficiency based on urine metabonomics
10.16438/j.0513-4870.2018-0479
- VernacularTitle:基于尿液代谢组学研究玄参对阴虚火旺甲亢大鼠的滋阴降火作用机制
- Author:
Ning ZHANG
1
;
Zi-hui LI
1
;
Hong-wei ZHAO
1
;
Mu PANG
1
;
Shu-min LIU
2
Author Information
1. Heilongjiang University of Chinese Medicine, Harbin 150040, China
2. Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine, Harbin 150040, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Radix Scrophulariae;
hyperthyroidism rat model of yin deficiency;
nourishing yin to reduce pathogenic fire;
metabonomics
- From:
Acta Pharmaceutica Sinica
2018;53(11):1843-1851
- CountryChina
- Language:Chinese
-
Abstract:
The Chinese herbal Radix Scrophulariae is the main medicine for nourishing yin and reducing fire. It can be used to treat hyperthyroidism due to yin deficiency and fire hyperactivity, but its mechanism is not clear. The present study was aimed to explore the mechanism of Radix Scrophulariae treatment of hyperthyroidism due to yin deficiency and fire hyperactivity. The urine metabolomic approach was conducted using the method of UPLC-TOF-MS. The results showed that Radix Scrophulariae has good therapeutic effects on hyperthyroidism rat model of yin deficiency. After treatment with Radix Scrophulariae, through metabolic profiling and protocol analysis, 6 potential metabolic markers may be closely related with the treatment mechanism of Radix Scrophulariae on this disease, including proline betaine, estrone, thymidine, 5-hydroxyindoleacetic acid, cyclic AMP and L-dopa. The strongest metabolic pathways were associated with tryptophan metabolism, pyrimidine metabolism, tyrosine metabolism, purine metabolism and steroid hormone biosynthesis. The urine metabolomic approach can be applied to clarify the therapeutic mechanism of Radix Scrophulariae on hyperthyroidism rat of yin deficiency, and provide the theoretical basis for the clinical practice of Radix Scrophulariae on nourishing yin to reduce pathogenic fire.
