Identification of metabolites of Reduning injection in rat plasma, bile, urine and feces after intravenous administration
10.16438/j.0513-4870.2018-0052
- VernacularTitle:静脉给药热毒宁注射液大鼠血浆、胆汁、尿液和粪便中代谢产物的鉴定
- Author:
Chun-xue WANG
1
;
Ling WANG
2
;
Da-wei QIAN
1
;
Huang-qin ZHANG
3
;
Wen-zhe HUANG
4
;
Tuan-jie WANG
4
;
Zhen-hua ZHU
2
;
Shu-lan SU
3
;
Sheng GUO
3
;
Jin-ao DUAN
2
Author Information
1. Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China
2. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China
3. National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
4. Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Reduning injection;
geniposide;
geniposidic acid;
secoxylogain;
chlorogenic acid;
metabolite
- From:
Acta Pharmaceutica Sinica
2018;53(7):1148-1155
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to determine the metabolites of Renduining injection in rats. The ultra-high performance liquid chromatography-LTQ Orbitrap mass spectrometric (UHPLC-LTQ-Orbitrap-MS) and mass defect filter techniques were applied to analyze the metabolites of Reduning injection in rat plasma, bile, urine and feces. As a result, we determined 14 metabolites of geniposide, including oxidation, dehydration, hydroxymethylene loss, hydrolysis, ring-opened, cysteine conjugation and glucuronidation conjugation of aglycone; 9 metabolites of geniposidic acid, consisting of dehydration, ring-opened, double-bond reduction and cysteine conjugation; 6 metabolites of secoxylogain including hydrolysis, hydroxymethylene loss, hydroxylation and ethylation; 12 metabolites of chlorogenic acid, containing decarboxylation, hydrolysis, methylation, acetylation, cysteinylglycine conjugation and glutathione conjugation. It provided information for the therapeutic effect of Reduning in vivo.
