Identification of chemical constituents in Naozhenning granule by UHPLC coupled with hybrid quadrupole-orbitrap MS
10.16438/j.0513-4870.2017-1190
- VernacularTitle:采用UHPLC-Q Exactive轨道肼高分辨质谱快速识别脑震宁颗粒的化学成分
- Author:
Shu-ting YU
1
;
Hai-xia LIU
2
;
Kun LI
2
;
Zheng-guo QIN
2
;
Xue-mei QIN
1
;
Ping ZHU
2
;
Zhen-yu LI
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China
2. Shanxi Zhendong Ante Biological Pharmaceutical Co., Ltd., Jinzhong 030600, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Naozhenning granule;
chemical constituent;
UHPLC-Q Exactive quadrupole-orbitrap MS
- From:
Acta Pharmaceutica Sinica
2018;53(4):609-620
- CountryChina
- Language:Chinese
-
Abstract:
Naozhenning granule, which is composed of 11 herbal drugs, is mainly used in the treatment of concussion, cerebral post-traumatic syndrome. As the chemical composition of Naozhenning was complex, the ultrahigh performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry was applied to identify the chemical constituents rapidly in the Naozhenning granule. By analyzing the retention behaviors, accurate molecular weight, the fragmentation pathway, and comparison with the MS data from the standards and references, as well as the automatic identification by the Compound Discover 2.0, a total of 161 compounds were identified or tentatively deduced, and the sources of constituents were also confirmed. The compounds identified in Naozhenning granule included 9 iridoids, 8 butylphthalides, 26 flavonoids and flavonoid glycosides, 8 phenolic acids, 8 monoterpenoid glycosides and 9 alkaloids, as well as the common compounds in the herbal drugs, such as organic acids, amino acids, and sugars. The chemical composition of Naozhenning was studied for the first time, which provides a scientific basis for the quality control and document of effective materials of Naozhenning granule.
