Comparative study on nucleosides in circulated Cordyceps sinensis of different sizes
10.16438/j.0513-4870.2017-0940
- VernacularTitle:基于核苷类成分对不同商品等级冬虫夏草的比较研究
- Author:
Li-li HUANG
1
;
Ke ZAN
2
;
Li-nong GUO
2
;
Jie LIU
2
;
Jian ZHENG
2
;
Shuang-cheng MA
2
Author Information
1. Guangxi Institute for Food and Drug Control, Nanning 530021, China
2. National Institutes for Food and Drug Control, Beijing 100050, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Cordyceps sinensis;
different sizes;
fingerprint chromatogram;
nucleosides
- From:
Acta Pharmaceutica Sinica
2018;53(4):598-603
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to establish a method to obtain the fingerprint chromatogram for the quantitative determination of Cordyceps sinensis in different sizes, a comparison of Cordyceps sinensis from five places was made to analyze its similarity and the content of main nucleosides (uridine, inoside, guanosine and adenosine). The assay was performed on a Waters XSelect HSS T3 C18 (4.6 mm×250 mm, 5 μm), with a mobile phase consisting of water (A)-acetonitrile (B) at the flow rate of 0.6 mL·min-1 (0-5 min, 0 B; 5-15 min, 0→10% B; 15-30 min, 10%→20% B; 30-33 min, 20%→50% B; 33-35 min, 50%→0 B; 35-40 min, 0 B). The detection wavelength was 260 nm and the column temperature was set at 30℃, and the injection volume was 5 μL. The results showed that there was no significant difference of the nucleosides in samples from the same place of the different sizes, but contents of the nucleosides variate a lot by production places. More data are required for further research. The method is proved for scientific and specific formulation of the standard in evaluation of circulated Cordyceps sinensis.
