Application of radical method to evaluation of antioxidant activity of Vleriana jatamansi Jones
10.16438/j.0513-4870.2017-0933
- VernacularTitle:蜘蛛香药材提取物抗氧化活性的研究
- Author:
Fei-fei WANG
1
;
Shou-hai WU
2
;
Yu-mei ZHANG
1
;
Shuang-cheng MA
1
;
Zhong DAI
1
Author Information
1. National Institutes for Food and Drug Control, Beijing 100050, China
2. The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Vleriana jatamansi Jones;
DPPH· free radical scavenging activity;
relationships between structures and activity
- From:
Acta Pharmaceutica Sinica
2018;53(3):439-443
- CountryChina
- Language:Chinese
-
Abstract:
The antioxidant activities of Vleriana jatamansi Jones were investigated and the relationship between the antioxidant effect and the chemical structure was explored. The free radical scavenging test, 2,2-diphenyl-1-picrylhydrazyl (DPPH•), was used to evaluate the antioxidant activities of the extracts of Vleriana jatamansi Jones with 0−100% menthol as extraction solvents. The polar and nonpolar HPLC conditions were conducted to isolate the main chemical compositions. The DPPH• tests were used in analysis of the free radical scavenging activities. Under polar HPLC separation conditions, 5 kinds of compounds were detected:chlorogenic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, hesperidin, and coffeic acid; under nonpolar HPLC separation conditions, acevaltrate, 1β-acevaltrate and valtrate were founded. Chlorogenic acid, 3,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid presented high DPPH• free radical scavenging activities. The results of antioxidant activity suggested that the coffee acyl from chlorogenic acid-like compounds had a high DPPH• free radical scavenging ability. Our investigation indicated that structure of the ortho hydroxyl phenol of chlorogenic acid-like compounds play a significant role in antioxidant activities. In addition, this work can also provide method and theory reference for improving the antioxidant activities of Vleriana jatamansi Jones.
