Construction and screening of phage display library for TIM-3 nanobody
10.16438/j.0513-4870.2017-1092
- VernacularTitle:TIM-3纳米抗体噬菌体展示文库的构建及筛选
- Author:
Lin-lin MA
1
;
Min ZHU
2
;
Guang-hui LI
2
;
Yan-fei LI
1
;
Jun-wei GAI
2
;
Ya-kun WAN
2
Author Information
1. Key Laboratory of Molecular Imaging of Shanghai Education Commission, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China
2. Shanghai Novamab Biopharmaceuticals Co., Ltd., Shanghai 201318, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
T cell immunoglobulin and mucin-domain-containing molecule-3;
nanobody;
phage display library
- From:
Acta Pharmaceutica Sinica
2018;53(3):388-395
- CountryChina
- Language:Chinese
-
Abstract:
Immunotherapy is a new strategy for cancer treatment that has the potential to treat all types of cancer. T cell immunoglobulin and mucin-domain-containing molecule-3 (TIM-3) is a key negative regulator of T cell activation. TIM-3 blockage using anti-TIM-3 monoclonal antibody therapy has a great appeal and special advantages. Nanobodies, derived from heavy chain fragment in camelid animals, are now proving clinical values in the development of antibody drugs. In this study, we have immunized camel with TIM-3 antigens and then constructed phage display library. Moreover, 29 nanobodies with different complementarity-determining regions sequences have been screened from the phage display library by phage display technology. In addition, we successfully constructed the cell line stably expressing TIM-3, and screened 10 TIM-3 nanobodies with high specificity and high affinity using flow cytometry. Our study will lay the foundation for the future screening and development of anti-TIM-3 whole humanized functional nanobody.
