The effects of catechin on inflammatory response induced by lipopolysaccharide in BV-2 cells
10.16438/j.0513-4870.2017-0993
- VernacularTitle:儿茶素对脂多糖诱导的BV-2细胞炎性反应的作用
- Author:
Qing WANG
1
;
Zhe-lin JIA
2
;
Li-juan SONG
1
;
Jie-zhong YU
3
;
Zhi-chao YANG
1
;
Wei-jia JIANG
1
;
Bao-guo XIAO
4
;
Cun-gen MA
1
Author Information
1. The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619, China
2. First Affiliated Hospital, Shanxi Medical University, Taiyuan 030001, China
3. Institute of Brain Science, Shanxi Datong University, Datong 037009, China
4. Institute of Neurology, Fudan University, Shanghai 200040, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
catechin;
lipopolysaccharide;
BV-2 cell;
inflammatory response
- From:
Acta Pharmaceutica Sinica
2018;53(2):244-248
- CountryChina
- Language:Chinese
-
Abstract:
The effects of catechin on inflammatory response of BV-2 cells were investigated using the lipopolysaccharide (LPS) model. BV-2 cells were incubated with LPS (1 mg·L-1) for 12 h in the microglia inflammatory model in vitro. After catechin and LPS co-incubation for 12 h, MTT, ELISA and Western blot were used to detect cell viability, cytokines, cell migration and protein expression. In addition, transwell assay was conducted to investigate the effect of catechin on cell chemokaxis. Catechin did not show any cytotoxicity effect on BV-2 cells, but reversed the change in cell morphology and inhibited the release of TNF-α and IL-1β, cell chemotaxis and phosphorylation of NF-κB/p65. In conclusion, Catechin could inhibit the LPS-induced inflammatory response in BV-2 cells.
