Celastrol-loaded macrophage membrane camouflaged PEG-PLGA nano-particles for targeted therapy of severe acute pancreatitis in rats
10.16438/j.0513-4870.2017-0846
- VernacularTitle:巨噬细胞膜包裹的PEG-PLGA载雷公藤红素纳米粒靶向治疗重症急性胰腺炎的大鼠体内药效学研究
- Author:
Wei-zhen QIAO
1
;
Xi CAO
1
;
Zhi-rong ZHANG
1
;
Tao GONG
1
;
Yao FU
1
Author Information
1. Key Laboratory of Drug Targeting and Drug Delivery Systems, West China School of Pharmacy, Sichuan University, Chengdu 610041, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
RAW264.7 macrophage;
poly(ethylene glycol) methyl ether-block-poly(lactic-co-glycolic acid);
nanoparticle;
celastrol;
severe acute pancreatitis
- From:
Acta Pharmaceutica Sinica
2018;53(1):127-132
- CountryChina
- Language:Chinese
-
Abstract:
Severe acute pancreatitis (SAP) is characterized by both local and systemic inflammatory responses. This study was designed to develop a site-specific delivery strategy for SAP therapy using celastrol (CLT). First, murine RAW264.7 cells were used as a model of macrophage cell line, cell membranes were obtained by emptying intracellular contents via hypotonic lysing, mechanical membrane disruption, and differential centrifugation. Poly(ethylene glycol) methyl ether-block-poly(lactic-co-glycolic acid) (PEG-PLGA) nanoparticles (NPs) were then prepared by sonication. With the collected membrane materials, macrophage membrane coated PEG-PLGA NPs (RNPs) were then prepared by extrusion through a 400 nm polycarbonate membrane. Biodistribution study in rats with SAP showed RNPs selectively accumulated in the inflamed pancreatic tissues. Compared with CLT loaded NPs, CLT loaded RNPs were proven to effectively attenuate local pancreatic inflammation and systemic inflammation in rats with SAP.
