Sophora alopecuroides ameliorates large intestine dampness-heat syndrome in rats by serum metabolomic approach based on UHPLC-Q/TOF-MS/MS
10.16438/j.0513-4870.2017-0735
- VernacularTitle:苦豆草治疗大肠湿热证大鼠血清代谢组学研究
- Author:
Xiao-song ZHANG
1
;
Qi MA
1
;
Yan-qiao WEN
1
;
Ya-hui ZHANG
1
;
Jian HE
1
;
Bo YIN
1
;
Wan-ling YAO
1
;
Zi-wen YUAN
1
;
Peng JI
1
;
Yong-li HUA
1
;
Yan-ming WEI
1
Author Information
1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Sophora alopecuroides;
large intestine dampness-heat syndrome;
serum;
metabolomics;
UHPLC-Q/TOF-MS/MS
- From:
Acta Pharmaceutica Sinica
2018;53(1):111-120
- CountryChina
- Language:Chinese
-
Abstract:
The Chinese herbal Sophora alopecuroides is widely used to clean intestine and eliminate dampness, and it has good therapeutic effects on treating bacillary dysentery and inflammatory bowel disease, etc. in clinics. However, the mechanism of treatment is not yet well understood. The present study was aimed to explore the mechanism of Sophora alopecuroides treatment of large intestine dampness-heat syndrome (LIDHS). The LIDHS model was performed by the comprehensive factors, including high temperature and humidity environment, high-sugar and high-fat diet, and intraperitoneal injection of Escherichia coli. The blood routine, serum proinflammatory cytokine levels and histopathological changes of intestine were detected and observed. Meanwhile, the serum metabolomic approach was conducted using the method of ultra performance liquid chromatography coupled to quadrupole time-of-flight mass/mass spectrometry (UHPLC-Q/TOF-MS/MS). The results showed that Sophora alopecuroides has good therapeutic effects on the LIDHS rat models. After treatment with Sophora alopecuroides, the abnormality of blood routine indexes as well as proinflammatory cytokines, including IL-1β, IL-2, IL-6 and TNF-α in vivo, tended to be normal, and the histopathological changes of intestine were improved. Through metabolic profiling and protocol analysis, 9 potential metabolic markers may be closely related with the treatment mechanism of Sophora alopecuroides on this disease, including taurine, L-tryptophan, LysoPE, LysoPC, LPA, DG, chenodeoxycholic acid disulfate, traumatic acid and 7-ketodeoxycholic acid, which were involved in taurine and hypotaurine metabolism, glycerophospholipid metabolism, glycerolipid metabolism, tryptophan metabolism and primary bile acid biosynthesis etc. The serum metabolomic approach can be applied to clarify the therapeutic mechanism of Sophora alopecuroides on LIDHS, and provide the theoretical basis for Sophora alopecuroides in clinical practice.
