Study of cancer cell apoptosis induced by Schizonepeta tenuifolia with microfluidic chip technology
10.16438/j.0513-4870.2016-0466
- VernacularTitle:基于微流控芯片技术的荆芥诱导肺肿瘤细胞凋亡谱效关系研究
- Author:
Jia-xin FAN
1
;
Shuai WANG
1
;
Xian-sheng MENG
1
;
Yong-rui BAO
1
;
Tian-jiao LI
1
Author Information
1. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Schizonepeta tenuifolia;
microfluidic chip;
A549 cell;
spectrum effect relationship;
grey correlation degree
- From:
Acta Pharmaceutica Sinica
2017;52(1):126-131
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to elucidate the chemical composition and anti-cancer effects of Schizonepeta tenuifolia's ethanol extracts. Microfluidic technology was used in the study of Schizonepeta tenuifolia from 9 different geographic regions. The ethanol extracts were examined with HPLC to establish their Fingerprints in order to analyze the relationship between the spectrum and efficacy index through Grey Correlation software, and a rapid HPLC-Q-TOF/MS method was established. The result shows that chromatographic peaks of the 19, 6, 11, 16, 18th are the representative diosmetin, luteoloside, hesperidin, luteolin, and apigenin. The 10, 12, 20th peaks may be naringenin-7-O-glucuronide or quercitrin, rosmarinate or acetylcorynoline, and 5,7-dihydroxy-6,4-dimethoxy flavone. The major chemical composition of Schizonepeta tenuifolia was found to have the anti-lung-tumor effects. A new method was established for the quality control of traditional Chinese medicine.
