Rapid identification of medicinal herbs through plant Direct-PCR
10.16438/j.0513-4870.2017-0426
- VernacularTitle:应用直接PCR技术的药用植物批量样本快速处理及分子鉴定
- Author:
Jin-Jia ZHANG
1
;
Hong XU
1
;
Shu-juan ZHAO
1
Author Information
1. The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Direct-PCR technology;
ITS;
DNA barcoding;
medicinal plant;
molecular authentication
- From:
Acta Pharmaceutica Sinica
2017;52(11):1763-1769
- CountryChina
- Language:Chinese
-
Abstract:
Direct-PCR technology was using a 15 minutes heat-lysis step instead of DNA extraction to get DNA templates with small amount of plant materials followed by sensitive PCR process to amplify target genes. In order to facilitate DNA barcoding in medicinal herb identification with Direct-PCR, we collected different tissues from 80 medicinal plants as material to amplify the ITS fragments. Through optimizing the PCR reaction, ITS of 80 plant samples was all successfully amplified. PCR products were sequenced and to do Blast analysis. These results suggest that Direct-PCR would improve the efficiency of DNA barcoding in the application of medicinal herb molecular authentication.
