Analysis of the Three Dimensional Structure of Envelope Protein of the Japnes Encephalitis virus Isolated in Korea.
- Author:
Jae hwan NAM
;
Soo Lim CHAE
;
Eung Jung KIM
;
Kyung Sik YOON
;
Ho dong LEE
;
Hae Wol CHO
;
Hyun Chul KOH
- Publication Type:Original Article
- MeSH:
Animals;
Encephalitis Virus, Japanese;
Encephalitis Viruses*;
Encephalitis Viruses, Tick-Borne;
Encephalitis*;
Guinea Pigs;
Korea*
- From:Journal of the Korean Society of Virology
1997;27(2):209-216
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Three dimensional structures of envelope protein from Korean isolates and Nakayama-NIH strain of Japanese encephalitis virus (JEV) were deduced by a computer program (HyperChem 4.0 Chemplus 1.0) based on the data of the three dimentional structure of Tick-borne encephalitis virus. In the three dimensional structure of envelope protein, neutralizing epitope and T-helper cell recognition site of C-terminal region of Korean isolates were structually similar to those of Nakayama-NIH but the N-terminal region was not. Korean JE isolates were compared with Nakayama-NIH strain by using cross-neutralization antibody test. Neutralizing activities of Korean isolates derived from guinea pigs were higher than those of Nakayama-NIH strain against Korean isolates, although the polyclonal antibody titers of Nakayama-NIH showed 1:160 to 1:640 against Korean isolates. According to the results from three dimentional structures and cross-neutralization analyses, the antigenic difference between Korean JE isolates and Nakayama-NIH strain may be dependent on structural difference of envelope protein.
