The effect of triptolide derivative LB-1 on imiquimod-induced psoriasiform inflammation of BALB/c mice
10.16438/j.0513-4870.2017-0683
- VernacularTitle:雷公藤甲素衍生物LB-1对咪喹莫特诱导的银屑病小鼠炎症的影响
- Author:
Fei NIU
1
;
Jing JIN
1
;
Qin ZHOU
1
;
Lin NI
1
;
Fang-fang LAI
1
;
Ming JI
1
;
Dong-ming ZHANG
1
;
Xiao-guang CHEN
1
Author Information
1. State Key Laboratory of Bioactive Substances and Functions of Nature Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
triptolide;
psoriasis;
imiquimod;
inflammation;
T-lymphocyte
- From:
Acta Pharmaceutica Sinica
2017;52(11):1692-1697
- CountryChina
- Language:Chinese
-
Abstract:
The aim of present study was to explore the effect of triptolide derivative LB-1 on imiquimod (IMQ) induced psoriasiform inflammation in BALB/c mice, and to investigate the immune mechanism of LB-1 in the prevention and treatment of psoriasis. In the present study, topical application of IMQ for seven days induced the psoriasiform inflammation in BALB/c mice. This is a promising mouse model of psoriasis for the natural immune reaction compared to those induced by xenograft, trangenic or gene knockout. psoriasis area and severity index (PASI) score, hematoxylin-eosin (HE) staining and flowcytometry were employed to investigate the changes of psoriasiform inflammation, histopathological response and percentage of T cells, respectively. The result showed that LB-1 significantly attenuated the psoriasiform inflammation. Com-pared with model group, PASI score were decreased in the LB-1 group. In the isolated immunocytes of spleen, LB-1 decreased percentage of CD8+ (P < 0.01) T cells and increased the ratio of CD4+/CD8+ T cells at the dosage of 2 mg·kg-1 (P < 0.01), whereas LB-1 raised percentage of CD4+ T cells and CD3+ T cells at the dosage of 4 mg·kg-1. In conclusion, the present study demonstrated that LB-1 attenuated psoriasiform inflammation induced by imiquimod in BALB/c mice. The mechanism of LB-1 action may be related to change percentage of CD4+ T, CD8+ T cells in the spleen. These results provide a basis for LB-1 or other triptolide derivative in the intervention of psoriasis in the future.
