Anti-inflammatory effect of TTF1-NP on lipopoiysaccharide stimulated human hepatocellular carcinoma cells
10.16438/j.0513-4870.2017-0432
- VernacularTitle:珍珠梅黄酮纳米粒抑制脂多糖诱发肝癌细胞的炎性作用
- Author:
Kui-yang SHAO
1
;
Xuan ZHANG
1
;
Wen-jun JIAO
1
;
Si-lin ZHANG
1
;
Xue-wu ZHANG
1
Author Information
1. Medical College, Yanbian University, Yanji 133002, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
5,2',4'-trihydroxy-6,7,5'-trimethoxy flavone nanoparticle;
liver cancer;
inflammation;
AKT/mTOR signaling pathway
- From:
Acta Pharmaceutica Sinica
2017;52(10):1549-1553
- CountryChina
- Language:Chinese
-
Abstract:
The study was designed to test the role of 5,2',4'-trihydroxy-6,7,5'-trimethoxy flavone nanoparticle (TTF1-NP) on lipopoiysaccharide (LPS)-induced inflammatory response, and to explore the anti-inflammatory mechanism in human hepatocellular carcinoma cells. Inflammatory responses were induced in human hepato-cellular carcinoma HepG2 cells with LPS; Proliferation effect of TTF1-NP in LPS-stimulated HepG2 cells were detected by MTT assay; The expression of TLR4, AKT/mTOR signaling related proteins and IL-6 were detected by Western blot assay. The results showed that TTF1-NP inhibited the proliferation of HepG2 cells induced by LPS in a dose-dependent manner; TTF1-NP inhibited the expression of TLR4, the activation of AKT and mTOR, and expression of IL-6 in a dose-dependent manner; TTF1-NP inhibited the activation of AKT/mTOR signaling pathway and TLR4 proteins leading to suppression of IL-6 expression in HepG2 cells stimulated by insulin. These results suggest that TTF1-NP inhibited inflammatory responses from LPS treatment with a potential mechanisms in the inhibition of AKT/mTOR pathway.
