Study of brain distribution of Ephedra alkaloids based on UHPLC-QTOF-MSE and UHPLC-MS/MS method
10.16438/j.0513-4870.2017-0262
- VernacularTitle:UHPLC-QTOF-MSE与UHPLC-MS/MS分析麻黄生物碱在血脑屏障间的移行及脑区分布特征
- Author:
Mi PENG
1
;
Dan SU
1
;
Han-yun LI
1
;
Bing-wei FENG
1
;
Peng-fei XU
1
;
Yong-gui SONG
1
Author Information
1. Jiangxi University of traditional Chinese Medicine, Nanchang 330006, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Ephedra sinica;
alkaloids;
central nervous system;
UHPLC-QTOF-MSE;
UHPLC-MS/MS
- From:
Acta Pharmaceutica Sinica
2017;52(9):1437-1443
- CountryChina
- Language:Chinese
-
Abstract:
Using ultra high performance liquid chromatography (UHPLC) coupled with mass spectrum (MS) technology, a method has been established for separation and analysis of alkaloid isomers. Alkaloids in Ephedra sinica transitionally crossed blood brain barrier (BBB) and the distribution were investigated. The concentrations of Ephedra alkaloids in rat central nervous system (CNS) were determined to acquire the distribution characteristics and differences in cerebral cortex, cerebellum, hippocampus, striatum, medulla oblongata and hypothalamus. It was founded that pseudoephedrine, norephedrine, norpseudoephedrine, methylephedrine (methylpseudoephedrine) were able to cross BBB fast via gastro-intestinal tract after administrated with Ephedra sinica. Cortex and hippocampus was the main distribution region, followed by hypothalamus, striatum and cerebellum, in which medulla oblongata had the least. The distribution of various alkaloids, as AUC0-t, brain/AUC0-t, blood was ephedrine > methylephedrine > demethyl ephedrine. Alkaloids in Ephedra sinica crossed BBB rapidly, showing the regional distribution tendency in central nervous system, and the distribution was diversity. This group of data provides distribution of bioactive constituents of Ephedra in CNS.
