Effect of Huangqin Tang on serum metabolic profile in rats with ulcerative colitis based on UHPLC-MS
10.16438/j.0513-4870.2017-0079
- VernacularTitle:基于UHPLC-MS研究黄芩汤对溃疡性结肠炎大鼠血清代谢谱的影响
- Author:
Dun-fang WANG
1
;
Yan-li WANG
1
;
Yi-wei WANG
2
;
Li-kun DU
3
;
Ying TONG
3
;
Xi CHEN
3
;
Shan-shan GUO
1
;
Hang-yu XU
1
;
Xu-ran MA
1
;
Tao LI
4
;
Wei-peng YANG
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. China Food and Drug Administration Institute of Executive Development, Beijing 100073, China
3. Heilongjiang University of Chinese Medicine, Harbin 150040, China
4. The Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Huangqin Tang;
metabolomics;
serum;
ulcerative colitis
- From:
Acta Pharmaceutica Sinica
2017;52(8):1306-1312
- CountryChina
- Language:Chinese
-
Abstract:
The study is aimed to test the effect of Huangqin Tang (HQT) on serum metabolic profile in rats with ulcerative colitis, and explore its possible action mechanism for ulcerative colitis (UC) rats. The model of UC rats with cell immunoreactivity was made using a compound method (trinitrobenzene sulfonic acid plus ethanol). Rats were randomly divided into the control group, the model group, and HQT group. Ultra performance liquid chromatography tandem mass spectrometry (UHPLC-MS), principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were employed to analyze the metabolic profile among normal group, the model group, HQT group. Potential biomarkers were screened in the serum based on the variable importance projection (VIP) value > 1, P< 0.05. As compared with the normal group, 16 potential biomarkers such as valine, tryptophan, lactic acid and urea were found and identified in the serum of model group rats. As compared with the model group, a part of the biomarkers were restored nearly to a normal state after HQT administration for 10 days. Metabolomic analysis revealed that the HQT has a certain therapeutic effect in UC rats, and the mechanism may be related to regulation of lipid metabolism, amino acid metabolism and energy metabolism.
