Preliminary study of lipid bilayer-coated calcium phosphate nanoparticles as a drug carrier for antitumor drug
10.16438/j.0513-4870.2017-0052
- VernacularTitle:脂质-磷酸钙核/壳纳米粒作为抗肿瘤药物载体的初步研究
- Author:
Yun-qiu MIAO
1
;
Shu-fang HE
2
;
Jin-ying LIANG
2
;
Qin KE
2
;
Xin-xin ZHANG
2
;
Rui WANG
1
;
Yong GAN
2
Author Information
1. School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
calcium phosphate;
nanoparticle;
paclitaxel;
drug release;
antitumor drug
- From:
Acta Pharmaceutica Sinica
2017;52(6):977-984
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to prepare lipid bilayer-coated calcium phosphate core-shell nanoparticles (LCAPNs), which can dissolve in an acidic environment to improve the tumor cell toxicity of antitumor drug. Paclitaxel (PTX) loaded lipid coated calcium phosphate nanoparticles (PTX-LCAPNs) were prepared by thin-film dispersion method. The morphology, particle size and in vitro release behavior were characterized. Meanwhile, the intracellular uptake, intracellular dissolution, cell toxicity of PTX-LCAPNs and intracellular accumulation of PTX were evaluated in human HCC cell line (Huh-7). The results suggested that the mean diameter of the spherical LCAPNs was 124.73±6.41 nm. The PTX-LCAPNs demonstrated little drug leakage in simulated normal physiological conditions, while a rapid release was observed in simulated intracellular condition in vitro. Moreover, the PTX-LCAPNs achieved 1.7 fold improvement in the intracellular PTX concentration leading to 5-fold reduction in half maximal inhibitory concentration (IC50) values of PTX compared with calcium phosphate nanoparticles loaded with PTX (PTX-CAPNs), demonstrating a stronger cancer cell lethality.
