Qualitative and quantitative analysis of major constituents of raw and processed Arctii Fructus by UHPLC-UV-Q-TOF-MS/MS
10.16438/j.0513-4870.2016-0967
- VernacularTitle:基于UHPLC-UV-Q-TOF-MS/MS的牛蒡子炒制前后化学成分定性定量研究
- Author:
Jing HU
1
;
Kun-ming QIN
2
;
Ting-ting ZHU
1
;
Xiao-li WANG
1
;
Meng-xue FAN
1
;
Bao-chang CAI
1
Author Information
1. Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China
2. Nanjing Haiyuan Prepared Slices of Chinese Crude Drugs Co. Ltd, Nanjing 210061, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Fructus Arctii;
processing;
UHPLC-Q-TOF-MS/MS;
HPLC-UV;
qualitative analysis;
quantitative analysis
- From:
Acta Pharmaceutica Sinica
2017;52(4):603-608
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we developed a qualitative analytical method based on liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) for identification of multi-constituents of raw Fructus Arctii (RFA) and processed Fructus Arctii (PFA). We established a UHPLC-UV analytical method for simultaneously determining 6 major compounds in Fructus Arctii. UHPLC- Q-TOF-MS/MS qualitative analysis was performed under negative and positive ion modes and a total of 23 chemical compounds were identified. The analysis data were subjected to a principle component analysis with a t-test. Ten peaks were found to be the main difference (P<0.05) between RFA and PFA. HPLC-UV quantitative method result showed the contents of 6 constituents were different between RFA and PFA. The results indicated that there was less arctiin, chlorogenic acid, isochlorogenic acid A in PFA than in RFA. However, there were higher levels of arctigenin, isochlorogenic acid B, isochlorogenic acid C in the PFA than RFA, which may be the main reason for different clinical efficacy of RFA and PFA.
