Endocytosis pathway and intracellular distribution of heparosan
10.16438/j.0513-4870.2016-1098
- VernacularTitle:Heparosan细胞入胞途径及其胞内分布
- Author:
Huan-huan PENG
1
;
Ying LI
1
;
Jia-yi YUAN
1
;
Jing-xiao CHEN
1
;
Jing-hua CHEN
1
Author Information
1. School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
heparosan;
endocytosis pathway;
intracellular distribution;
drug carrier
- From:
Acta Pharmaceutica Sinica
2017;52(3):474-480
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the endocytosis pathway of heparosan and its intracellular distribution were investigated in MCF-7 tumor cells and COS7 normal cells. The endocytosis inhibition and cellular probe location experiments showed that MCF-7 tumor cells took heparosan more efficiently and selectively than COS7 cells. The cellular uptake of heparosan was energy-dependent in both MCF-7 tumor cells and COS7 normal cells. Moreover, the major endocytosis pathway of heparosan into MCF-7 tumor cells was caveolin-mediated endocytosis and macropinocytosis. The internalized heparosan was mainly located in lysosomes of the cells.
