Chromatographic fingerprint analysis of Toosendan Fructus by HPLC-CAD coupled with chemometrics methods
10.16438/j.0513-4870.2016-0965
- VernacularTitle:HPLC-CAD结合化学计量学的川楝子饮片指纹图谱研究
- Author:
Chun-ni ZHANG
1
;
Ying-zi WANG
1
;
Xin-guang SUN
2
;
Yang ZHAO
2
;
Wei ZHENG
3
;
Wen-hua LI
1
;
Zhen LONG
4
;
Bai-ping MA
2
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
3. Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
4. ThermoFisher Scientific Corporation, Beijing 100080, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Toosendan Fructus;
HPLC-CAD;
fingerprint;
similarity evaluation;
hierarchical cluster analysis;
principle components analysis
- From:
Acta Pharmaceutica Sinica
2017;52(3):456-461
- CountryChina
- Language:Chinese
-
Abstract:
A new method was developed for the chromatographic fingerprint analysis of Toosendan Fructus by HPLC coupled with the charged aerosol detector (CAD) in the present study. Samples were well separated on an Agilent ZOBAX SB C18 column (4.6 mm×250 mm, 5 μm) by gradient elution using acetonitrile and water containing 0.1% formic acid (v/v) at the flow rate of 1.0 mL·min-1. The column temperature was 30℃ and the injection volume was 5 μL. The nitrogen inlet pressure of the charged aerosol detector (CAD) was 35 psi, and the nebulizer chamber temperature was 35℃. In addition, the method of the chromatographic fingerprints combined with multivariate statistical analysis was effective and reasonable lead to an accurate classification of 20 batches of samples from different locations. The results showed that 28 common peaks were observed in the fingerprint and the samples were classified into three clusters. The established method was well validated, and showed high precision, good repeatability, and satisfactory stability. It may serve in the quality control and evaluation of Toosendan Fructus.
