In vivo study of Chuankezhi metabolism in rat
10.16438/j.0513-4870.2016-0955
- VernacularTitle:喘可治注射液在大鼠体内的药物代谢研究
- Author:
Qian-ru FENG
1
;
Jie-jing YU
1
;
Jing-jing ZHAN
1
;
Yi-juan XU
2
;
Yeuk-lung CHOW
1
;
Shun-jun XU
2
;
Liu YANG
1
Author Information
1. Guangdong Provincial Hospital of Chinese Medicine/Guangdong Academy of Chinese Medical Sciences, Guangzhou 510120, China
2. Guangzhou ImVin Pharmaceutical Co., Ltd., Guangzhou 510663, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Chuankezhi injection;
flavonoid;
liquid chromatography-tandem mass spectrometry;
drug metabolism
- From:
Acta Pharmaceutica Sinica
2017;52(3):449-455
- CountryChina
- Language:Chinese
-
Abstract:
To study the metabolic products of main compounds of Chuankezhi injection in rat, 12 Sprague Dawley rats were classed into 2 groups, a blank control group and an intermuscular administration group, respectively. Rat feces and urine samples were collected from 0-24 h and 24-48 h after administration. All the samples were ultrasonically treated with methanol and then analyzed using LC-LTQ Orbitrap MSn. By comparison with the total ion chromatogram of samples from the blank control group, the metabolites in the samples of drug-treated group were screened. These metabolites were further analyzed by multistage product ion scanning and comparison of retention time with reference substances. As a result, a total of 12 flavonoid metabolites were tentatively identified from the rat feces and no metabolite was discovered in the rat urine. Epimedin C and icariin were detected in the rat blood samples after 30 min of administration, but their metabolites and other original flavones were not detected. Furthermore, no original flavones and their metabolites were detected in rat blood samples after 2 and 4 h of administration. The potential metabolism paths were further characterized and the principal in vivo transformation of flavones from Chuankezhi injection were deglycosylation, dehydration, methylation, oxidation and isomerization in rats.
