Activating blood biological potency assay and chemical fingerprint chromatogram applied to quality evaluation of rhubarb
10.16438/j.0513-4870.2016-0896
- VernacularTitle:基于活血生物效价和化学指纹图谱的大黄品质评价研究
- Author:
Hai-zhu ZHANG
1
;
Peng TAN
2
;
Zhen-jie LIU
3
;
Jian WANG
4
;
Jia-bo WANG
3
;
Xiao-he XIAO
3
Author Information
1. Department of Pharmacy and Chemistry, Dali University, Dali 671000, China
2. Chengdu Institute for Food and Drug Control, Chengdu 610045, China
3. China Military Institute of Chinese Materia Medica, 302 Military Hospital, Beijing 100039, China
4. College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
rhubarb;
activate blood and remove stasis;
biological potency assay;
fingerprint chromatogram;
quality evaluation
- From:
Acta Pharmaceutica Sinica
2017;52(3):436-442
- CountryChina
- Language:Chinese
-
Abstract:
The biological potency assay and chemical fingerprint chromatogram were applied to quality evaluation of rhubarb. Using the biological potency as indicators, we evaluated the differences in quality of multiple batches of rhubarbs and related products. Using the platelet aggregation analyzer, we determined platelet aggregation rate in the different rhubarbs preparations, and calculated the biological potency based on the simplified probit principle. UPLC was adopted to establish the fingerprint spectra for rhubarbs. The spectral efficiency correlation analysis between chromatograms and biological potencies were conducted using the double variables of SPSS 22.0 software. We used three chemical composition to verify the potency. The biological potency results suggest that Rheum palmatum has a more potent activity than Rheum tanguticum, and wine-treated rhubarb had a higher potentcy than charred. We identified 10 elements in the Fingerprint Spectrum. The relevant elements including rhein-8-O-β-D-glucoside, emodin-8-O-β-D-glucoside and rhein have the strongest activity in the inhibition of platelet aggregation. In conclusion, this study provides a analytical method for rhubarb biological potency based on determination of the maximum antagonism rate model. The rhein may be the effective substance. It may serve as a reference in the quality control of wine processed rhubarb products.
