Investigation of the potentially effective components of Semen Ziziphi Spinosae based on “in vitro to in vivo” translation approach
10.16438/j.0513-4870.2016-0644
- VernacularTitle:基于体内-体外物质组关联分析酸枣仁潜在效应物质
- Author:
Yan YAN
1
;
Qiang LI
1
;
Chen-hui DU
2
;
Jin-ping JIA
3
;
Hong-xia FENG
2
;
Xue-mei QIN
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University of Traditional Chinese Medicine, Taiyuan 030619, China
2. College of Chinese Materia Medicine, Shanxi University of Traditional Chinese Medicine, Taiyuan 030619, China
3. Scientific Instrument Center, Shanxi University, Taiyuan 030006, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Semen Ziziphi Spinosae;
"in vitro to in vivo" translation approach;
high resolution mass spectrometry;
effective and active constituent
- From:
Acta Pharmaceutica Sinica
2017;52(2):283-290
- CountryChina
- Language:Chinese
-
Abstract:
The study aims to investigate the effective components of Semen Ziziphi Spinosae (SZR) in nourishing the heart and tranquilizing the mind. A method of ultra high liquid chromatography (UHPLC) coupled with Q Exactive high resolution mass spectrometry (HR-MS) was developed. Based on the UV spectra, retention time and MS spectra, 25 compounds of SZR extract were identified or tentatively characterized, including 12 flavonoids, 8 triterpenoids saponins, 2 fatty acid and 3 alakoids. The study illuminated the major chemical components. Twenty bioactive components were determined in rat urine after oral administration of SZR extract by "in vitro to in vivo" translation approach, including 16 prototype compounds and 4 metabolites. Spinosin, swertisin, jujuboside A and B were considered as the effective and active constituents in SZR of the sedative and hypnotic effects, which emodies characteristics of multiple components. It was beneficial exploration for searching the effective and active constituents of SZR in nourishing the heart and tranquilizing the mind.
