Comparative study of the corticosterone and glutamate induced PC12 cells depression model by 1H NMR metabolomics
10.16438/j.0513-4870.2016-0543
- VernacularTitle:谷氨酸和皮质酮诱导的PC12抑郁症细胞模型差异性的1H NMR代谢组学研究
- Author:
Xiao-yan HE
1
;
Jian-li CHEN
1
;
Huan XIANG
2
;
Yao GAO
1
;
Jun-sheng TIAN
1
;
Xue-mei QIN
1
;
Guan-hua DU
3
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
2. Physical Education Departments, Shanxi University, Taiyuan 030006, China
3. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
metabolomics;
corticosterone;
glutamate;
PC12 cell;
depression
- From:
Acta Pharmaceutica Sinica
2017;52(2):245-252
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to analyze the change of metabolites in the PC12 cells and its medium induced by corticosterone (CORT) and glutamate (Glu) by proton nuclear magnetic resonance (1H NMR) metabolomics. The multivariate statistical analysis was employed to identify the difference between control groups and induced groups, respectively. In addition, metabolite pathway analysis was performed to explore the characteristic of CORT-induced and or Glu-induced PC12 cells depression model, and to provide the references for the selection of in vitro depression models as well as the further understanding of the mechanism on depressive disorders. We found 36 differential metabolites in CORT-induced PC12 cells and medium and 42 in Glu-induced PC12 cells. Furthermore, correlation analysis results show that serine and 2-oxoisoleucine were associated with most differential metabolites in CORT-induced PC12 cells. Lactate and glutathione were significantly correlated to the vast majority of differential metabolites in Glu-induced PC12 cells. We speculated that CORT-induced PC12 cell models may affect the fatty acid metabolism and cell membrane structure, and Glu-induced PC12 cell models may have a difference in the glycolysis and antioxidants.
