Anti-tumor effect and impact on tumor immune microenvironment of tumor-targeted Salmonella VNP20009
10.16438/j.0513-4870.2016-0425
- VernacularTitle:肿瘤靶向性沙门氏菌VNP20009抗肿瘤作用及其对肿瘤免疫微环境的影响
- Author:
Yu-heng HAN
1
;
Xing-huan LAI
1
;
Zi-wei LE
1
;
Zi-chun HUA
1
Author Information
1. The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Salmonella;
tumor microenvironment;
melanoma;
tumor-infiltrating lymphocyte;
monocyte;
T-lymphocyte
- From:
Acta Pharmaceutica Sinica
2016;51(9):1417-1422
- CountryChina
- Language:Chinese
-
Abstract:
Salmonella is a gram-negative bacterium that has an ability of tumor-targeting growth and proliferation. Attenuated Salmonella VNP20009 is a virulence genes-knockout bacterial strain based on Salmonella typhimurium, and it has an advantage of good therapeutic effect and low toxicity. One of the mechanisms of anti-tumor effect of VNP20009 is the induction of inflammatory reaction within tumor tissues. We used B16F10 melanoma model to investigate the mechanism of the anti-tumor effect of VNP20009. VNP20009 treatment effectively inhibited tumor growth and promoted the apoptosis and necrosis of tumor cells. VNP20009 increased the accumulation or infiltration of CD8+ T cells and CD11b+ monocytes within tumor tissue by raising the level of immune response and thus, induce the production of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) to kill tumor cells by breaking the immuno-evasion barrier in the tumor microenvironment.
