Analysis of distribution, phenotype and clinical correlation of CD163 positive macrophages in osteoarthritis
10.16462/j.cnki.zhjbkz.2019.07.022
- Author:
Wen-ming WANG
1
;
Li WANG
;
Hui CHU
;
Ying-bo ZHOU
;
Xi-fu SHANG
;
Xiao-mei LI
Author Information
1. Department of Rheumatology and Immunology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, China
- Publication Type:Research Article
- Keywords:
Osteoarthritis;
Macrophages;
Inflammation;
IL-10;
TNF-α
- From:
Chinese Journal of Disease Control & Prevention
2019;23(7):856-860
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to analyze the distribution characteristics of CD163+ macrophages in peripheral blood and synovium of patients with osteoarthritis (OA)，and investigate the correlation of CD163+ macrophages, intracellular tumour necrosis factor(TNF-α), interleukin-10 (IL-10) expression with the disease duration, inflammation level and body mass index (BMI). Methods The percentages of CD163+ macrophages in synovium and peripheral blood and their intracellular levels of TNF-α and IL-10 were detected by flow cytometry. Meanwhile, immunofluorescence was uesed to observe the distribution of CD163+ macrophages in synovial tissue of patients with OA or fracture. Results (1) There were significant differences in the percentage of CD163+ macrophages in synovial and peripheral blood between OA and fracture patients(all P<0.05). (2) The expression of TNF-α in CD163+ macrophages of synovium and blood of OA patients with disease duration below 10 years was significantly higher than that of patients with OA disease duration above 10 years (all P<0.05) . The expression of IL-10 was significantly lower than that of patients with OA disease duration above 10 years (all P<0.05). (3) C-reactive protein (CRP) was positively correlated with BMI in patients with OA disease duration below 10 years (r=0.680， P<0.001), while showed no correlation with BMI in patients with OA duration above 10 years(r=0.084， P=0.187). (4) Immunofluorescence confocal microscopy showed a large number of CD163+ macrophages invaded around the synovial vessel of patients with OA. Conclusion The distribution of CD163+ macrophages in synovium and peripheral blood of OA patients with different course of disease was different, and showed differentiated cell subtypes.
