Overexpression of NtPMT and HnH6H changed hyoscyamine-rich Atropa belladonna to scopolamine-rich varieties
10.16438/j.0513-4870.2016-0275
- VernacularTitle:转NtPMT和HnH6H转变莨菪碱型颠茄为东莨菪碱型颠茄
- Author:
Hong QUAN
1
;
Ke XIA
2
;
Jun-lan ZENG
2
;
Min CHEN
3
;
Xiao-zhong LAN
4
;
Zhi-hua LIAO
2
Author Information
1. Institute of Plateau Ecology, Tibet Agricultural and Animal Husbandry College, Nyingchi 860000, China
2. School of Life Sciences, Southwest University, Chongqing 400715, China
3. College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China
4. Medicinal Plants Research Center, Tibet Agricultural and Animal Husbandry College, Nyingchi 860000, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Atropa belladonna;
scopolamine;
putrescine-N-methyltransferase;
hyoscyamine-6β-hydroxlase;
metabolic engineering
- From:
Acta Pharmaceutica Sinica
2016;51(12):1913-
- CountryChina
- Language:Chinese
-
Abstract:
Atropa belladonna L. is the commercial plant material for production of tropane alkaloids, including hyoscyamine and scopolamine. The wild-type Atropa belladonna is characterized by the hyoscyaminerich chemotype, in which the hyoscyamine content is much higher than the scopolamine content. It is the common goal for the pharmaceutical industry to increase the content of scopolamine in A. belladonna. Based on the T0 progeny of transgenic A. belladonna with NtPMT and HnH6H overexpression, T1 progeny of transgenic A. belladonna were obtained through self-pollination and used in a field trial. The 461 bp fragment of NtPMT and the 1 077 bp HnH6H were simultaneously expressed from T1 progeny of transgenic A. belladonna, but were not obtained from the wild-type A. belladonna. At the transcription level, the expression of NtPMT and HnH6H were detected in T1 progeny of transgenic A. belladonna, but were not detected in the wild-type plants. Further, the alkaloids were analyzed by HPLC. In the stems and leaves of T1 progeny of transgenic A. belladonna, hyoscyamine was not detected and scopolamine was detected at very high levels; in the stems and leaves of wild-type A. belladonna, hyoscyamine was detected at much higher levels. In the leaves of T1 progeny of transgenic A. belladonna, the content of scopolamine was 15-36 folds higher than that of wildtype leaves; in the stems of T1 progeny of transgenic A. belladonna, the scopolamine content was 37-108 folds higher than that of wild-type stems. In conclusion, overexpression of NtPMT and HnH6H greatly enhanced conversion of hyoscyamine into high-value scopolamine and improved the commercial value of A. belladonna.
