Determination of hepatic and small intestinal distribution of lignans of Wuzhi tablet in rats by liquid chromatography tandem mass spectrometry method
10.16438/j.0513-4870.2016-0526
- VernacularTitle:LC-MS/MS法测定五酯片中木脂素活性成分在大鼠肝脏及肠道的分布
- Author:
Xiao-ling QIN
1
;
Wen-hai DUAN
1
;
Hai-fan WANG
1
;
Ying WANG
2
;
Xiao CHEN
3
;
Min HUANG
4
;
Hui-chang BI
4
Author Information
1. Guangdong Food and Drug Vocational College, Guangzhou 510520, China
2. The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
3. The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
4. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Wuzhi tablet;
lignan;
tissue distribution;
liquid chromatography tandem mass spectrometry
- From:
Acta Pharmaceutica Sinica
2016;51(12):1891-
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to determine the hepatic and small intestinal distribution of active lignans in rats after treated with Wuzhi tablet (WZ, Schisandra sphenanthera extract) by LC-MS/MS method. Male Sprague-Dawley rats were sacrificed at 0.25, 1.5, 4, 6, 10, 24 h after an oral administration of WZ, and then hepatic and small intestinal samples were collected for analysis. The results showed that concentrations of lignans in liver and small intestine of rats were decreased with WZ pretreated time. The concentrations of all lignans in rat liver and small intestine at 0.25 h were the highest after a single oral administration. All lignans was undetectable in all tissues 24 h after oral dosing, suggesting lignans of WZ were eliminated rapidly in rats. The concentrations of schisandrin A, schisandrol B and schisantherin A in small intestine were much higher than those in the liver, suggesting the effect of WZ on the intestinal metabolism enzyme might be more potent than that on the liver. In short, the current results suggest that lignans of WZ were not accumulated in rat liver and small intestine. The concentrations of lignans of WZ in small intestine were much higher than those in liver.
