Apoptosis of human hepatocellular carcinoma HepG-2 cells upon inhibition of STAT3 by 5,2',4'-trihydroxy-6,7,5'-trimethoxy flavone nanoparticle
10.16438/j.0513-4870.2016-0540
- VernacularTitle:珍珠梅黄酮纳米粒抑制人肝癌HepG-2细胞STAT3诱导细胞凋亡
- Author:
Bin XIAO
1
;
Xuan ZHANG
1
;
Mei-lan ZHANG
1
;
Xue-wu ZHANG
1
Author Information
1. Yanbian University Medical College, Yanji 133002, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Sorbaria sorbifolia;
nanoparticle;
hepatocellular carcinoma;
signal transducer and activator of transcription 3;
cellular apoptosis
- From:
Acta Pharmaceutica Sinica
2016;51(12):1845-
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to investigate the mechanism of 5,2',4'-trihydroxy-6,7,5'-trimethoxy flavone nanoparticle (TTF1-NP) in the induction of apoptosis of human hepatocellular carcinoma HepG-2 cells. MTT assay, immunocytochemical staining and flow cytometry with Annexin V-FITC/PI were used to demonstrate inhibition of proliferation of HepG-2 cells and cell apoptosis. The inhibition was studied in a dose- and time-dependent manner. Western blot results showed that TTF1-NP down-regulated the signals of survivin, p-STAT3 and STAT3, but up-regulated the expression level of cleaved caspase-3. Taken together, our results showed that TTF1-NP induced HepG-2 cell apoptosis through inhibition of the STAT3 expression.
