Application of high resolution melting curve to identification of Cimicifugae Rhizoma
10.16438/j.0513-4870.2016-0216
- VernacularTitle:高分辨率熔解曲线技术在多基原药材升麻鉴定中的应用
- Author:
Wei SUN
1
;
Chao XIONG
1
;
Jing-jian LI
2
;
He-gang LIU
3
;
Zhi-gang HU
1
;
Di LIU
3
;
Wei-chao REN
1
;
Shi-lin CHEN
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
3. College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Cimicifugae Rhizoma;
high resolution melting;
identification of traditional Chinese medicinal materials;
ITS2
- From:
Acta Pharmaceutica Sinica
2016;51(10):1638-
- CountryChina
- Language:Chinese
-
Abstract:
High-resolution-melting analysis (HRM) is a new technology derived from qPCR and is widely used in the study of polymorphism, genotyping, and single nucleotide mutation. Advantages of HRM include cost-effectiveness and time-efficiency over PCR-based genotyping. However, the application of HRM in the authentication of herbal products is still limited with few studies on the classification and identification of herbal products. In this study, Cimicifugae Rhizoma was used as an example to verify the stability and accuracy of HRM technique in identification of Chinese materia medica. HRM assay was established for identification based on ITS2 region of Cimicifugae Rhizomas and its adulterants (including 41 samples). Our findings showed that HRM allows not only the identification of adulteration but also the quantification of the most common admixture. This study is significant for better quality in the verification of the authenticity of herbal medicine. The method is promising for future identification of traditional Chinese medicinal materials.
